PCR Training Program

A. Safety operation in a PCR lab

  • Hazardous chemicals.
  • Safety rules for machines operation.
  • Reagent preservation and lab security

B. Basic theory of PCR

  • DNA structure.
  • The function of DNA Polymerase.
  • The principle of polymerase chain reaction (PCR).
  • Basic requirements and design of PCR lab.
  • Potential problems of PCR.

C. Introduction of PCR related reagents:

  • Nucleic acid extraction kit.
  • IQ2000/IQREAL/IQ Plus /POCKIT Reagent Set.

D. Introduction of PCR related equipment

E. Review of some major diseases

F. Basic theory of viral disease prevention

  • Avoid vertical transmission.
  • Avoid horizontal transmission.

A. Practice of DNA extraction

B. Practice of PCR operation procedure

  • Reaction condition.
  • Reagents preparation.
  • Reaction Procedure.

C. Practice of gel electrophoresis if needed:

  • Agarose gel preparation.
  • Electrophoresis.
  • Gel staining and data assay.

D. Sampling procedure and sample preservation

  • Preservation of samples.
  • Preservation of nucleic acid samples.
  • Introduction of sample dissecting tools.
  • Disinfection procedure of the sample dissecting tools.
  • Sampling strategy.

A. Perform the assay by using real samples

  • To compare the sensitivity, reproducibility, and to practice more, it is necessary to go through the whole procedure by real samples again.
  • Positive standards and negative control are required.
  • Requirements of sample.

B. Detail description of PCR diagnostic procedure

  • Sampling, sensitivity, and statistical issue.
  • False positive or false negative result.
  • Reproducibility.
  • Lab management.

C. How to integrate the diagnostic results into real practice.

A. Real samples operation: to confirm the operation and process the other diagnostic systems.

B. Discussion.