Introduction
PCR technique has been commonly employed in the aquaculture industry for disease diagnosis and prevention. But with the growing demand for a quantitative research tool and requirement for a high throughput screening system, real-time PCR is receiving more attention and becoming more important in the industry.
IQ REAL™ KHV Quantitative System is a real-time based diagnostic system which not only meets the above demands but its advanced designs, listed below, also provides the shrimp culture industry a more accurate, more sensitive, and more flexible real-time system.
Advantages of IQ REAL™ KHV Quantitative System:
- TaqMan® assay strategy: This system can tolerate higher concentration of sample load than SYBR Green. Consequently, it is able to detect the samples with very low viral/shrimp cell ratio.
- Dual color design: This system can quantify both viral (FAM) and shrimp genomic nucleic acid (VIC) by a single reaction. This built-in internal control avoids the false negative results from failed extraction or inhibition.
- Absolute and comparative quantitation: This system can quantify the viral amount by both absolute quantitative method (Qab, viral amount/μl) and comparative quantitative method (Qcp, viral amount/ 10,000 shrimp cells). The Qcp method can reflect the real infectious level and provide constant result for a sample within wide range of concentration.
- No standard required: There is no need to make a calibration curve for Qcp method. A unique ΔΔCt strategy is introduced to interpret the final result. There is no waste for standards as the reagents are only for the use of samples.
*TaqMan® is the trademark licensed under Applied Biosystems.
