IQ2000 YHV/GAV Detection and Prevention System
Introduction
In 1992, Yellow-head virus (YHV) was first discovered in Thailand on black tiger prawn (Penaeus monodon) though it is now known to have caused extensive losses to the east coast of the Gulf of Thailand early in 1991. This disease affects juvenile to sub-adult prawn and is usually characterized by a pale to yellowish coloration of the cephalothorax and gills, and erratic swimming of infected animal near the surface at the pond edge. Gill associated virus (GAV), which morphologically resembles YHV, has also been reported in Penaeus monodon from Australia, and has been associated with sporadic outbreaks of disease since 1996.
The IQ2000™ YHV/GAV Detection and Typing System for penaeid shrimps is a commercialized molecular biology system for direct detection of these two similar viruses. It can detect and differentiate the prototype of these two viruses and provide semi-quantitative results plus build-in internal control system. The knowledge about the nature of YHV and GAV and their RNA sequences which the system was based upon were originally developed by CSIRO, Australia, and BIOTEC, Thailand. A licensing agreement was signed in 2001 between GeneReach and CSIRO/BIOTEC to commercialize this technology for the manufacturing of YHV/GAV detection system.
According to the recent research results, other YHV/GAV related viruses have also been found in some Asian countries. Due to the lack of sequence information, IQ2000™ YHV/GAV Detection and Typing System may not be able to detect these YHV/GAV related viruses, or may cross-react with these viruses. The impact and severity of these viruses on shrimp farming and their relationship with YHV/GAV is still under investigation.
Specifications:
￭ Packing size: 200 reactions.
￭ Detection Limit: 10 copies/reaction.
￭ Quantitative capability: 3 different levels of infection can be differentiated.
￭ Sample throughput: for 40 samples, from sample preparation to final results require 4.5 to 5 hours.
￭ Built-in internal control system: eliminate false negative results from failed extraction or reaction.
￭ Quantified positive standard: monitor the sensitivity of detection.
￭ Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.
An example of the results is shown and explained below:
Lane 1: YHV P(+) standard, 2,000 copies/reaction
Lane 2: YHV P(+) standard, 200 copies/reaction
Lane 3: YHV P(+) standard, 20 copies/reaction
Lane 4: GAV P(+) standard, 2,000 copies/reaction
Lane 5: GAV P(+) standard, 200 copies/reaction
Lane 6: GAV P(+) standard, 20 copies/reaction
Lane 7: Sample of severe YHV infection
Lane 8: Sample of light YHV infection
Lane 9: Sample of severe GAV infection
Lane10: Sample of light GAV infection
Lane11: YHV/GAV negative sample
Lane M: Molecular weight markers, 848 bp, 630 bp, 333 bp
Applications:
￭ This reagent is intended for testing fresh, frozen, and ethanol-preserved samples, e.g. PL and gill.
￭ Ideal for the specific pathogen-free (SPF) animal selection, evaluation of culture environment, grow-out monitoring, and quarantine service.
