Taura Syndrome Virus (TSV) was first reported in the Gulf of Guayaquil, Ecuador in 1992 and has caused severe impact on the L. vannamei culture industry since then. This virus is widely seen in America as well as Asia, particularly in Taiwan and China as a result of improper introduction of L. vannamei brood stocks in the late 1990s. The disease phases of this positive sense, single strained piconavirus, could be classified into 3 stages. In the acute phase, the diseased animals show a red discolouration in the tail and appendages and usually die whilst molting. The mortality rate is estimated at 80% to 85%. Shrimps that survive the molting process come to the recovery phase or the chronic phase. In the chronic phase, infected shrimps appear and behave normally despite remain diseased.

PCR technique has been commonly employed in the aquaculture industry for disease diagnosis and prevention. But building up a PCR lab is expensive and technician required. These make PCR detection limited in big farms. In order to provide pond-side PCR detection system for everyone in need, we introduced an innovative system - IQ Plus into the market. IQ Plus system using a new technology – insulated isothermal PCR (iiPCR), which is a compact and portable system and can detect TSV within 1 hour. The extraction procedure has been simplified to an easy way for everyone can easily perform the assay. IQ Plus system would be a useful tool for aquaculture pathogen detection and prevention anytime anywhere.